Part:BBa_K4131000
AccD1 gene (C. glutamicum), codon optimized for E. Coli expression
The acetyl-CoA carboxylase of C. glutamicum is composed of a biotin carboxylase (AccBC), a biotin carboxyl carrier protein (AccE), and a transcarboxylase (AccD1). They carboxylated acetyl-CoA into malonyl-CoA, which is necessary for the elongation of nascent fatty acid. The carboxylation occurs in two steps. In the first step, the ATP-dependent biotin carboxylase (AccBC) catalyzes the transfer of a carboxyl group to biotin, which is linked to the biotin carboxyl carrier protein (AccE). In the second step, the transcarboxylase (AccD1) transferred the carboxyl group from carboxybiotin to acetyl-CoA to form malonyl-CoA.
In our project, the AccD1 is co-expressed with AccBC and AccE to form the acetyl-CoA carboxylase and enhance the malonyl-CoA production.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 280
- 1000COMPATIBLE WITH RFC[1000]
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